Effect of Aegiceras corniculatum against MCP-1 dependent monocyte chemotaxis: A novel therapeutic approach to target Atherosclerosis

Effect of Aegiceras corniculatum against MCP-1 dependent monocyte chemotaxis: A novel therapeutic approach to target Atherosclerosis

Session: 
Medicinal Chemistry
Author(s): 
RAZZAK Anam - Molecular Pathology, Department of Pathology, Dow diagnostic Reference and Research Laboratory, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Talat ROOME - Molecular Pathology, Department of Pathology, Dow diagnostic Reference and Research Laboratory, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Ayaz AHMED - Molecular Pathology, Department of Pathology, Dow diagnostic Reference and Research Laboratory, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Saeed KHAN - Molecular Pathology, Department of Pathology, Dow diagnostic Reference and Research Laboratory, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Rafiq KHANANI - Molecular Pathology, Department of Pathology, Dow diagnostic Reference and Research Laboratory, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Ahsana DAR - H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan
Muhammad Iqbal CHAWDARY- H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan

Aegiceras corniculatum (Linn) Blanco is a mangrove plant that has been traditionally used in folklore medicine against cardiovascular and rheumatic inflammatory diseases. We evaluated the anti-inflammatory effect of ethyl acetate extract of A. corniculatum against MCP-1 dependent cell migration by  mouse peritonial inflammation using both in vivo and adoptive transfer model. Our results reveal that ethyl acetate extract (50 mg/kg) reduced monocyte migration by 90% in oral treatment and >70% inhibitory effects in adoptive transfer model at  50 µg/ml indicates that it impairs MCP-1 dependent cell migration via cPLA2 and iPLA2 signaling pathways. Moreover A. corniculatum extract (10µg/ml) showed tremendous inhibitory effects against super oxide anion (O2.-) production in PMN and opsonized zymosan-stimulated human neutrophil upto 80%. With  these exciting findings we are able to identify the bio diverse properties of A. corniculatum extract with less toxic effects as remarkable contribution in the field of medicine and therapeutics.