Comparative Phytochemical Analysis of Chinese and American Starvine by HPTLC and HPLC

Comparative Phytochemical Analysis of Chinese and American Starvine by HPTLC and HPLC

Session: 
Poster Session
Author(s): 
LYLES, James T. - Bent Creek Institute, 100 Fredrick Law Olmsted Way, Asheville, NC 28806, USA
Paula TYLER - Center for the Study of Human Health, Emory College of Arts and Sciences, 550 Asbury Circle, Candler Library 107, Atlanta, GA 30322, USA
Kevin SPELMAN - Health, Education and Research in Botanical Medicines, 2209 Dollarhide Way, Ashland, OR 97520, USA
Stephanie T. PIERCE - Department of Environmental Studies, Emory College of Arts and Sciences, 400 Dowman Drive. Math and Science Center 5th Floor E510. Atlanta, GA 30322, USA
Carl BROWN - Department of Environmental Studies, Emory College of Arts and Sciences, 400 Dowman Drive. Math and Science Center 5th Floor E510. Atlanta, GA 30322, USA
Cassandra QUAVE - Department of Dermatology, Emory University School of Medicine and Center for the Study of Human Health, Emory College of Arts and Sciences, 550 Asbury Circle, Candler Library 107, Atlanta, GA 30322, USA

A comparative HPTLC and HPLC-PDA analysis was performed on alcoholic extracts of Schisandra chinensis (Turcz.) Baill. (Chinese Starvine, CS) and Schisandra glabra (Brickell) Rehder (American Starvine, AS) fruits. Endemic to the Southeastern United States, it is suspected that AS was significant to indigenous Native American populations. Although there is little information concerning the uses of AS, CS has a long and well-documented ethnobotanical history. In this study, we investigated schisandrol A, schisandrol B, schisandrin A, schisandrin C, γ-schisandrin and schisantherin A in both species and quantified four compounds using standards. CS had much higher levels of schisandrol A (7.3% of total crude extract) than AS (0.26%), AS had higher levels of schisandrol B (6.7% vs. 1.6% in CS) and schisandrin A (4% vs. 2.1%), and roughly equal levels of γ-schisandrin (1.6% vs. 2.0%). This analysis represents an important step in assessing the future significance of AS for traditional medicine and conservation initiatives.